Document 0556
 DOCN  M9480556
 TI    [A synthetic peptide-based immunoenzyme test system for the joint
       detection of human retrovirus (HTLV-1 and HIV-1) antibodies]
 DT    9410
 AU    Rasuli AM; Klepikov NN; Gurtsevich VE; Andreev SM; Meshcheriakova DV;
       Rasuli ShM
 SO    Gematol Transfuziol. 1993 May;38(5):36-9. Unique Identifier : AIDSLINE
       MED/94307568
 AB    The study was made to develop an immunodiagnostic test system based on
       synthetic peptides able to detect in the same assay the total
       concentration of heterospecific antibodies against human retroviruses
       HTLV-1 and HIV-1. Three panels of reference-sera contained antibodies to
       HTLV-1 (70 specimens), HIV-1 (50 and 16 specimens) and 4 synthetic
       peptides corresponding to protein fragments p19 gag and gp46 env HTLV-1,
       gp120 and gp41 env HIV-1. Immune reactivity of the peptides with
       reference sera was measured in the immunoassay. It is established that
       relevant peptides mimic immunodominant B-epitopes of structural proteins
       HTLV-1 and HIV-1 and are recognized specifically by relevant antiviral
       antibodies. The enzyme immunoassay test system has been designed using a
       peptide combination in a single antigen complex. The system showed high
       diagnostic sensitivity.
 DE    Antibodies, Heterophile/*BLOOD  Antigenic Determinants  Comparative
       Study  English Abstract  Human  HIV Antibodies/*BLOOD  HIV-1/*IMMUNOLOGY
       HTLV-I Antibodies/*BLOOD  *Immunoenzyme Techniques  Peptide
       Fragments/*DIAGNOSTIC USE/IMMUNOLOGY  Reference Values  Sensitivity and
       Specificity  JOURNAL ARTICLE

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